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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Signal. 2014 Sep 16;26(12):2912–2920. doi: 10.1016/j.cellsig.2014.09.005

Fig. 2.

Fig. 2

Selective inhibition of HDAC1 and HDAC2 is sufficient to stimulate SUMOylation in cardiac myocytes and cardiac fibroblasts. (A) Selectivity profiles for the indicated HDAC inhibitors are shown; X = inhibited. (B) NRVMs were stimulated with phenylephrine (PE) in the absence or presence of the indicated HDAC inhibitors for 48 hours; DMSO vehicle (Veh.; 0.1% final concentration) was used as a negative control. SUMO-1 conjugates were examined by immunoblotting. (C) Unstimulated NRVMs were treated for 48 hours with the indicated HDAC inhibitors prior to immunoblotting for SUMO-1 conjugates. (D) Neonatal rat ventricular fibroblasts were serum-starved for 24 hours prior to treatment with the indicated HDAC inhibitors for 48 hours. (E) Independent plates of adult rat ventricular fibroblasts were serum starved for 24 hours prior to treatment with MGCD. SUMO-1 conjugates were resolved on a 7.5% polyacrylamide gel as opposed to (A – D), which employed 10% polyacrylamide gels.