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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Signal. 2014 Sep 16;26(12):2912–2920. doi: 10.1016/j.cellsig.2014.09.005

Fig. 3.

Fig. 3

HDAC2 regulates cardiac protein SUMOylation. (A) NRVMs were treated with MGCD0103 or BA60 for 48 hours, and protein homogenates were immunoblotted with two distinct antibodies that detect free RanGAP1 (lower band) and SUMO-1-ylated RanGAP1 (upper band). (B) NRVMs were infected with lentiviruses encoding shRNAs directed toward HDAC1, HDAC2 or HDAC3. shControl is an shRNA that is predicted to fail to target any mammalian mRNA transcript. After 96 hours of infection in the presence of PE, cells were lysed and HDAC1, HDAC2, HDAC3, SUMO-1, and RanGAP1 were detected by immunoblotting, as indicated.