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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Oct 2;1839(11):1330–1340. doi: 10.1016/j.bbagrm.2014.09.015

Fig. 1.

Fig. 1

Identification of the proximal promoter of PRMT5. (A) Two types of PRMT5 promoters cloned from LNCaP genomic DNA with indicated SNPs and an indel, as well as a series of 5′-truncated promoters were used to construct luciferase reporter genes. (B and C) The indicated reporter genes in A were co-transfected with pRL-TK into LNCaP and PC-3 cells for 24 hours for measurement of the luciferase activities. Results were obtained from at least three independent experiments in triplicate, and were normalized to the vector control (Basic). (*, p<0.05; Student’s t test). (D) Luciferase activities of 5′-truncated reporter genes (B6 and B7) in LNCaP, PC-3 and A549 cells. Results from 4–6 independent experiments are presented as mean ± SEM. Statistical significance (**, p<0.01 and ****, p<0.0001) was determined when compared with B7 by one-way ANOVA followed by Dunnett’s test.