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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Surg Res. 2014 Jul 2;192(2):254–262. doi: 10.1016/j.jss.2014.06.054

Figure 4. CTF and intracellular pathway.

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

Figure 4

[A] EGFR phosphorylation in response to CTF [10 nM] and EGF [10μg/dL] in the presence and absence of the pertussis toxin [PTx] or Gαq inhibitor [GP-2A]. Preincubation with bARKCT resulted in a decreased EGFR phosphorylation in response to CTF. EV, empty vector. Values are the mean±s.e.m. ratio of active /total EGFR protein for five experiments [**p<0.01]. Representative western blots are shown [B] and [C].

[D] Inhibition of intracellular calcium [BAPTA-AM], src [PP2] and PKC [H7 and GFX] blocked EGFR activation in response to CTF [10 nM] and EGF [10 μg/dL]. Values are the mean±s.e.m. ratio of active /total EGFR protein for five experiments **p<0.01]. Representative western blots are shown [E] and [F].

[G] Inhibition of oxygen free radical production through either the general inhibitor NAC and the more specific inhibitors DPI and apocynin also decreased EGFR activation in response to CTF [10 nM] and EGF [10 μg/dL]. Values are the mean±s.e.m. ratio of active /total EGFR protein for five experiments [**p<0.01]. Representative western blots are shown in [H].