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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Oct 9;9(2):605–617. doi: 10.1016/j.celrep.2014.09.004

Figure 1. Adipose tissue macrophages (ATMs) physically interact with CD4+ T cells in an antigen-dependent manner.

Figure 1

(A) Immunofluorescence analysis of MHCII+ cells (red) in FALCs (Fat-Associated Lymphoid Clusters) regions of mouse adipose tissue.

(B) Immunofluorescence analysis of HLA-DR+ (red) cells in human omental tissue. Representative images presented from similar results from 5 independent samples.

(C) Maximum intensity projection of z-stacks in FALCs in eWAT from CD11c-mCherry mice adoptively transferred with CFSE-CD4+ cells.

(D) Time-lapse images of interacting CD11c+ ATMs (red) and OTII CD4+ T cells (green) in eWAT from CD11c-mCherry mice injected with BSA (upper) or OVA (lower). Imaging of adipose tissue performed with intravital multi-photon confocal microscopy over a 20 minute imaging window.

(E) CD4+ T cell migration pathways in adipose tissue from mice injected with BSA (upper) or OVA (lower).

(F) Velocity and (G) displacement lengths of individual CD4+ T cells in eWAT.

(H) Percent of CD4+ T cells interacting with CD11c+ cells in eWAT. Microscopy data are representative of three independent experiments.

Data are means ± SEM. ** p < 0.01, *** p <0.001 versus BSA.