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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Cell Rep. 2014 Oct 9;9(2):605–617. doi: 10.1016/j.celrep.2014.09.004

Figure 3. Generation of macrophage-specific MHCII knockout (MMKO) mice.

Figure 3

(A) Representative flow cytometry plots of MHCII-expressing cells in eWAT SVCs. ATMs (CD45+ CD64+) (upper panels) and ATDCs (CD45+ CD64- CD11c+) (lower) from control (CON) and MMKO mice.

(B) Quantitation of ATMs and ATDCs (upper) and frequency of MHCII+ cells in ATMs and ATDCs (lower) from CON and MMKO mice. 5 mice assessed per group in 4 independent cohorts.

(C) Immunofluorescence images showing loss of MHCII+ expression in (green) and resident Mgl1+ ATMs (red). Scale bar = 50 μm.

(D) qPCR analysis of eWAT from CON and MMKO mice

(E) Quantitation of total blood myeloid cells and total lymphocytes.

(F) Frequency of CD4+ and CD8+ T lymphocytes in thymus, spleen and mesenteric lymph nodes (mLN).

Data are means ± SEM. ***, p <0.001 vs. CON.