Figure 6. Macrophage-specific MHCII deficiency reduces obesity-induced CD4⁺ ATT accumulation and maturation.

Quantitation of (A) CD3⁺ ATTs and (B) CD4⁺ and CD8⁺ ATTs in eWAT.

(C) Quantitation of Tconvs and Tregs in eWAT expressed as % SVCs (left) and as % CD4⁺ ATTs (right) in CON and MMKO.

(D) Frequency of proliferating Ki67⁺ CD4⁺ ATTs in eWAT.

(E) Representative flow cytometry plots gated on CD3⁺ CD4⁺ cells to quantify effector-memory (E/M; CD44^high CD62L^-), central-memory (C/M; CD44^high CD62L⁺) and naïve (CD44^low CD62L⁺) CD4⁺ ATT subsets in eWAT.

(F) Quantitation of naïve CD4⁺ ATT subsets in eWAT.

(G) Quantitation of E/M CD4⁺ ATT subsets in eWAT.

(H) Ifng gene expression in FACS-sorted CD4⁺ ATTs from HFD-fed CON and MMKO.

Data are means ± SEM. * p <0.05, ** p <0.01, *** p<0.001 versus CON.