Figure 6. hES cells retain Tra-1-60 expression following SFK inhibition.
The H1 line of hES cells was grown in mTeSR self-renewal medium or switched to differentiation medium in the absence (DMSO carrier) or presence of the pan-SFK inhibitor, A-419259, at the concentrations shown. Cells were fixed and immunostained for the cell-surface pluripotency marker Tra-1-60 three days later (magnification 400×). This experiment was repeated three times with comparable results; representative fields of cells from each condition are shown.