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. 2014 Dec 3;4:7242. doi: 10.1038/srep07242

Figure 1. CH25H and its products suppress HCV infection.

Figure 1

A: Schematic diagram of FACS analysis of HCV-GFP in total, RFP+, RFP- populations. Relative replication of HCV-GFP reporter virus is defined as GFP+% x the geometric mean fluorescence intensity (MFI). B: Contour maps of HCV-GFP replication in Huh7.5.1 cells transfected with the indicated RFP-ISG. C: Effect of overexpression of RFP-CH25H on HCV-GFP replication in total, RFP- positive or negative population normalized to vector control. HCV-GFP was quantified by the calculated value of the percentage % GFP-positive population and MFI of GFP signal. D: Medium was collected from HEK293T cells after 48 h transfection with indicated expression vectors. Freshly plated Huh7.5.1 cells were treated with the conditioned medium for 8 h and then infected with HCV-GFP at 0.2MOI. HCV-GFP was quantified by FACS at 48 hpi. E: Huh7.5.1 cells were pretreated with increasing amounts of 25HC (0, 1, 5 uM) for 8 h. HCV-GFP infection and quantification were measured at 48 and 72 hpi. All experiments were performed in triplicates and data shown are representative of three independent experiments with SEM indicated by error bars. *P < 0.05; **P < 0.01; ***P < 0.001.