Skip to main content
. 2014 Dec 3;4:7242. doi: 10.1038/srep07242

Figure 5. CH25H disrupts NS5A dimer formation.

Figure 5

A: Co-IP assays using Flag control vector or Flag-tagged full-length NS5A as bait to capture YFPn-tagged NS5A domain I were performed in the presence of an increasing amount of HA-tagged CH25H, HA-tagged CH25H-M, or HA-tagged CH25H truncation mutant constructs. Captured proteins were analyzed by Westernblot using anti-GFP and anti-Flag antibodies. All results were confirmed by three independent experiments. All the gels were run under the same experimental conditions as detailed in the Methods section, and full-length blots were cropped for final display. Full-length blots are included in the supplementary information. Effects of NS5A or NS4A on antiviral activities of CH25H (B) or CH25H-M (C). The indicated plasmids expressing control, NS5A or NS4A expression plasmids were cotransfected with CH25H wt or mutant CH25H expression plasmids into Replicon cells. HCV RNA level was measured by real-time RT-PCR 48 hours after transfection. All experiments were performed in triplicates and data shown are representative of three independent experiments with SEM indicated by error bars. *P < 0.05.