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. 2014 Dec 3;4:7235. doi: 10.1038/srep07235

Figure 4. LCN-2 expression in the B16-F1 and NIH-3T3 cells cultured under hypoxic conditions.

Figure 4

B16-F1 and NIH-3T3 cells were cultured for the indicated times under normoxic or hypoxic conditions. (a) HIF-1α protein expression was determined by western blotting. β-Actin was used as a loading control. Full-length blots/gels are presented in Supplementary Figure 2. LCN2 (b) and CA IX (c) mRNA levels were quantified by real-time RT-PCR. Data show relative mRNA expression using β-actin mRNA as an internal standard. Values represent the means of 3 individual experiments. Bars represent standard deviations. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. normoxic conditions.