Figure 7. NF-κB activity in B16-F1 cells was unchanged under hypoxic conditions.

NF-κB activity was investigated by dual luciferase assay (Promega) using B16-F1 cells cotransfected with plasmid DNAs expressing the firefly luciferase gene containing the NF-κB response element and the Renilla luciferase gene. Values represent the means of 3 individual experiments. Bars represent standard deviations.