Skip to main content
. 2014 Dec 3;4:7300. doi: 10.1038/srep07300

Figure 2. AFSM adapters and primers.

Figure 2

(a, c) Sequences of three different types of double-stranded AFSM adapters. The barcode adapter terminates with a 5-bp barcode at the 3′ end of its top stand and a 5-bp overhang at the 5′ end of its bottom strand that is complementary to the sticky end generated by EcoRI. The HpaII-methylation adapter has a HpaII-compatible sticky end, and the MspI-methylation adapter has a MspI-compatible sticky end. (b) PCR primer sequences for EcoRI-HpaII. (d) PCR primer sequences for EcoRI-MspI.