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. 2014 Dec;160(Pt 12):2807–2819. doi: 10.1099/mic.0.081836-0

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© 2014 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Purification of recombinant All1371 and gel filtration. (a) SDS-PAGE (15 %) analysis of samples from the various purification steps performed after affinity chromatography. Lane 1, cell-free extract of recombinant E. coli, 15 µg protein; lane 2, flow-through, 15 µg protein; lane 3, All1371 after elution, 2 µg; lane M, protein standard. (b) All1371 was subjected to gel filtration calibrated with protein standards (filled diamonds; see Methods for details). The elution profile (indicating the elution position of All1371, arrow) and SDS-PAGE results of the obtained fractions are shown in the inset.