Table 1. Biochemical and kinetic properties of All1371.
Measurements were performed at 28 °C, pH 7.5; n≥3; 100 % = 107.1 U mg−1.
| Property | Value (mean±sd) | |
| Molecular mass (kDa) | Native complex* | 39 |
| Monomer, apparent† | 26.0 | |
| Monomer, calculated‡ | 26.6 | |
| Oligomeric structure | Monomer or homodimer | |
| KM (mM) | Glucose | 0.118±0.01 |
| KM (µM) | Polyphosphate | 1.76±0.26 |
| vmax (U mg−1) | Glucose, polyphosphate | 107.1±15.3 |
| kcat (s−1) | Glucose, polyphosphate | 48.2±6.9 |
| K0.5 (mM) | Mannose | 24.3±2.36 |
| vmax (U mg−1) | Mannose | 0.43±0.04 |
| kcat (s−1) | Mannose | 0.21±0.017 |
| Phosphoryl donor specificity (% vmax) | Polyphosphate | 100 |
| ATP, GTP, UTP, CTP, ADP, AMP | 0 | |
| Pyrophosphate | 0 | |
| Without polyphosphate | 0 |
*
Determined by size exclusion chromatography.
†
Determined by SDS-PAGE.
‡
Calculated according to the primary structure, including the linker peptide of the GST tag.