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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: Dev Biol. 2014 Aug 30;395(2):379–389. doi: 10.1016/j.ydbio.2014.08.027

FIG. 1.

FIG. 1

A conserved, endothelial-specific enhancer in the ECE1 locus. (A) VISTA plot comparing human and mouse sequence within the ECE1 locus (upper schematic). Human sequence from hg19 (Build GRCh37.p13, Chr1: 21606465-21606628) is used as the reference sequence. CNS, conserved, non-coding sequence; UTR, untranslated exon sequence. A 623-bp fragment encompassing the CNS containing the FOX:ETS motif was cloned upstream of the hsp68 minimal promoter for expression of E-galactosidase (lower panel). (B) ClustalW multiple sequence alignment of the core 164-bp ECE1 enhancer region from human, mouse, and opossum. Conserved nucleotides are indicated with asterisks. The conserved FOX:ETS motif, a second conserved ETS site, and the conserved SOX site are indicated.