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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: Dev Biol. 2014 Aug 30;395(2):379–389. doi: 10.1016/j.ydbio.2014.08.027

FIG. 3.

FIG. 3

ECE1 enhancer activity is restricted to arterial endothelium at E12.5. Immunostaining of transverse sections through the trunk region of ECE[623]-lacZ transgenic embryos collected at E12.5. Endogenous Ece-1 protein expression (A) is present in arteries and veins; β-galactosidase protein expression from the ECE1[623]-lacZ transgene (Ac) is restricted to arteries. β-galactosidase protein expression (Bc,Cc) overlaps with expression of the arterial markers Nrp1 (B) and Cx40 (C). β-galactosidase expression (D’,E’) is found only in arteries and is mutually exclusive from the expression of the venous markers Nrp2 (D) and COUP-TFII (E). Respective merged images for each of the markers are shown in A″-E″. A, artery, V, vein. Scale bars, 100 μm.