Figure 6. Overexpression of NFATc1 in MC38^Par cells increases tumor incidence and liver metastases.

(A) Western Blot showing NFAT proteins in MC38^Par cells transfected with either empty vector (VEC) or NFATc1, β-actin is used as a loading control and Ramos extract as positive control. (B) Analysis of NFATc1 mRNA in MC38^Par cells shown in (A). (C) Rates of trans-endothelial invasion for MC38^Par cells shown in (A–B). Individual replicate wells from a representative experiment are plotted with the mean and the standard error of the mean (bars and whiskers). (D) Representative mice from splenic metastasis model (n=12–14/group) using MC38^Par cells shown in (A–D). (E) Bioluminescence of mice injected with MC38^Par cells shown in (A–D) at 14^th days. (F) Incidence of liver metastases for mice injected at day 14. (G) Liver weight to body weight ratio in mice injected with either MC38^Par shown in (A–F) cells at day 14 post-injection. *p<0.02, *** p=0.0004, **** p<0.0001.