Figure 7.

HIV-1 virion production is dependent on glycolysis. A. Infectivity of culture supernatants from HIV-1 NL4.3 infected primary CD4+ T cells, Jurkat (Jur) and CEM-ss (CEM) cells that were cultured for 24 hours in media containing 1 g/L glucose or galactose, was determined by β-galactosidase activity of the indicator cell line TZM-bl. B. Western blots showing the presence of p24^Gag in the supernatant from cultures of HIV-1 NL4.3 infected primary CD4+ T cells , Jurkat and CEM-ss in cultures containing 1 g/L galactose (gal) or glucose (glc). C. Quantified data from western blotting of p24^Gag shown in (B). All experiments were performed in triplicate and are shown as the average normalised to the infectivity of cultures containing glucose. Error bars represent the standard deviation.