Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cancer Res. 2014 Oct 6;74(23):7014–7023. doi: 10.1158/0008-5472.CAN-14-1220

Figure 2. Nocifensive behavior over time in bone cancer model.

Figure 2

Time-course of spontaneous pain-related behaviors following injection of either NCTC 2472 cells in Hanks balanced salt solution (HBSS) (open circles, n=36) or HBSS (sham) (inverted triangles, n=8) into the mouse femur show that spontaneous nocifensive behavior is temporally separated into “surgical (orthopedic) pain” (Day 1-7) and “bone cancer” pain (Day 7-30). Spontaneous nocifensive behavior exhibited by HBSS-injected (sham) mice show that by Day 7 post-surgery, spontaneous pain-related behaviors approach those exhibited by naïve mice (open squares, n=8). By Day 10 post-cancer-cell injection, time spent in nocifensive behavior by sarcoma-injected mice is easily differentiated from that of sham-injected mice. Error bars represent SEM.