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. 2013 Nov 8;77(2):198–208. doi: 10.1111/tpj.12378

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© 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Production of EPA and DHA in C. sativa. (a) Biosynthetic pathway for the production of the LC-PUFAs EPA and DHA, with the various enzyme activities shown in different colours [also used in (b)]. (b) Simplified maps of vectors p5_EPA and p7_DHA used for transformation of C. sativa. Abbreviations: Cnl, conlinin 1 promoter for the gene encoding the flax 2S storage protein conlinin; USP, promoter region of the unknown seed protein of Vicia faba; SBP, sucrose binding protein 1800 promoter; NP, napin; OtΔ6, Δ6-desaturase from O. tauri; TcΔ5, a Δ5-desaturase from Thraustochytrium sp.; Piw3, ω3-desaturase from Phytophthora infestans; PsΔ12, a Δ12-desaturase from Phytophthora sojae; EhΔ4, Δ4-desaturase from E. huxleyi; PSE1, a Δ6-elongase from P. patens; OtElo5, Δ5-elongase from O. tauri; OCS, 35S, E9 and CatpA represent terminators.