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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Signal. 2014 Oct 5;27(1):90–103. doi: 10.1016/j.cellsig.2014.09.019

Figure 10. kB2R agonist pre-treatment inhibits kB1R-mediated effects on endothelial permeability.

Figure 10

HLMVEC, grown to confluence on gold electrodes coated with 10 μg/ml fibronectin, were cytokine pre-treated (10ng/ml IL-1β + 100u/ml IFN-γ, 16 h). Medium was changed, cells were allowed to stabilize, and all samples to be treated with BK were pretreated for 20 min with 40 μM MGTA to inhibit carboxypeptidase conversion to DABK. A. Cells were treated with 1) 1 μM DAKD; 2) 1 μM BK for 10 min, then 1 μM DAKD; 3) 1 μM BK and TER was measured. B. Cells were treated with 1) 1 μM DAKD + 200 μM pyrogallol; 2) 1 μM BK for 10 min, then 1 μM DAKD + 200 μM pyrogallol; 3) 1 μM BK + 200 μM pyrogallol and then TER was measured. Results show mean values ± S.E. for n = 4.