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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Signal. 2014 Oct 5;27(1):90–103. doi: 10.1016/j.cellsig.2014.09.019

Figure 4. kB2R agonist downregulation of the kB1R calcium response requires the expression of kB2R.

Figure 4

A. HEK cells stably expressing only kB1R were first stimulated with kB2R agonists BK or KD, followed by kB1R agonists DABK or DAKD and the increase in [Ca2+]i was recorded. The traces are representative from at least three experiments. B. HEK cells stably expressing only kB1R were pretreated for 10 min with vehicle (△) or 1μM BK (▲), followed by various concentrations of kB1R agonist DAKD, or stimulated only with various concentrations of kB2R agonist BK (●). The increase in [Ca2+]i was recorded and quantified using the area under the curve as in Fig. 3. The data are representative of three experiments. C. HEK cells only stably expressing kB1R were pretreated with various concentrations of kB2R agonist BK for 10 min, and then stimulated with different concentrations of kB1R agonist DAKD and the increase in [Ca2+]i was recorded and quantified as in B. The data shown are mean ± SE (n=3).