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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Cell Signal. 2014 Oct 5;27(1):90–103. doi: 10.1016/j.cellsig.2014.09.019

Figure 5. kB2R agonists induced co-endocytosis of kB2R and kB1R.

Figure 5

HEK cells stably expressing kB1R and kB2R were incubated with 1 μM kB2R agonists KD or BK, kB1R agonist 1 μM DAKD or kB1R antagonist des-Arg10-HOE 140 (DA-HOE 140) for 10 min at 37 °C. After acid washing (or not, as indicated), the cells were further incubated with 4 nM [3H]DAKD (A) or [3H]BK (B) for 90 min on ice and radiolabel binding to kB1R or kB2R was determined as described in Methods. The data in A and B were calculated as percent of total binding compared with the cells which were not treated with agonist or antagonist, and shown as mean ± SE (n=3). *P<0.05 vs control not treated by agonist or antagonist; #P<0.05 vs KD or BK (ANOVA followed by Tukey’s test).