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. 2013 Oct 28;164(2):233–244. doi: 10.1111/bjh.12622

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© 2013 Celgene Corporation. British Journal of Haematology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig 2 — The CRBN65 antibody detects recombinant and endogenous CRBN protein with high sensitivity. (A) Recombinant human full-length CRBN (0·2–60 ng) along with DF15 and DF15R cell extracts (20 μg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and probed with CRBN65 antibody (1:1000). (B) Linear regression analysis using CRBN signal as measured on LI-COR Oddysey for rhCRBN is linear in the 0·2–60 ng range, and CRBN protein measured in DF15 cells falls within linear range of detection. CRBN, cereblon; kDa, kiloDalton; Mol W, molecular weight; R², coefficient of determination.