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. 2014 Jan 8;15(2):197–211. doi: 10.1111/tra.12139

Figure 9.

Figure 9

Simultaneous depletion of Hrs and CD63 prevents the formation of small ILVs. HeLa cells were depleted of Hrs or CD63 alone or Hrs and CD63 and were incubated in the presence of EGF and anti-EGFR gold for 25 min prior to fixation. Cells were analysed by conventional EM (A). Binning the ILV diameters to either <40 nm or >40 nm revealed that simultaneous CD63 depletion prevented the increase in number of smaller ILVs/MVB induced by Hrs depletion (B). Data shown are the mean ± SD (>40 MVBs/condition, 2 independent experiments). Scale bar: 200 nm.