Figure 1.

PEG purification of a library of DNA origami objects. a) Illustration (RR to 100 hb) and CanDo-computed²⁰ models (plate1 to 42 hb_b) of a panel of DNA objects comprising a variant of Rothemund’s single-layer DNA rectangle (RR)^2a and five multilayer objects (four-, ten-, 18-, or 42-helix bundle in honeycomb lattice design; 100-helix bundle in square lattice design),⁹ a plate-like object with aperture and double-stranded loop (plate1),^4c a plate-like object (plate2),^4c an asymmetric 82-helix bundle in square lattice design (pointer),^2h a gear-like object (gear),¹⁸ a flexible hinged-bar object (hinge),¹⁸ a letter-A-like object (“A”),^20b a straight and bent version of a robot-like object (robot, robot_b),²⁰ a letter-S-like object (“S”),^20b a bow-like object (bow),^20b and bent versions of an 18-helix bundle and a 42-helix bundle (18hb_b, 42hb_b).^{2c, 18} The library of objects thus samples a wide spectrum of shapes, aspect ratios, and mechanical properties. b) Images of agarose gels on which unpurified self-assembly reaction mixtures (U) and PEG purified samples (P) of the objects listed above in (a) were electrophoretically separated. Labels: po, gel loading pocket; m, properly folded monomers; ex, non-integrated excess staple strands. c) Exemplary TEM micrographs of single particles in unpurified reaction mixtures (U) and PEG-purified samples (P). Scale bars: 50 nm.