Figure 3.

Preparation of solid material and dense solutions containing intact DNA objects. a) Approximately 6.8 mg of solid material containing a variant of Rothemund′s rectangle (unlabeled). b) Approximately 5.5 mg of solid material containing 42-helix bundle objects, labeled each with one ATTO655 dye. c) Approximately 8.4 mg of solid material containing 24-helix bundle objects, labeled each with ten cyanine-3 dyes on average. d) Left: unpurified self-assembly reaction mixture containing approximately 50 nm of folded 24-helix bundle objects and 150 nm excess staple strands; center: dense solution containing approximately 5.6 μm of folded 24-helix bundle objects, prepared by redissolving the materials from (c); right: reference sample containing 70 μm of a cyanine-3 modified DNA oligonucleotide. e–g) TEM micrographs of dried and redissolved single-layer rectangle (e), 42-helix bundle (f), and 24-helix bundle (g); insets: average single-particle micrographs obtained from non-dried (left) and dried and redissolved samples (right). Scale bars: 50 nm (field-of-view micrographs), 10 nm (insets).