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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Int J Biochem Cell Biol. 2014 Sep 16;55:279–287. doi: 10.1016/j.biocel.2014.09.007

Figure 2. S100A8/A9 up-regulation inhibited motility and matrigel invasion.

Figure 2

(A) KB, KB-EFGP and KB-S100A8/A9 cells migrate to fill a uniform gap in the monolayer. Cells were treated with Mitomycin-C before the motility assay to inhibit cell proliferation. Monolayer closure expressed as a function of time after removal of uniform swath of cells was observed, photographed, and measured at times up to 48 h. The experiments were performed in triplicate for each cell line and repeated at least three times with similar results. (B) Matrigel invasion capacity of KB, KB-EFGP and KB-S100A8/A9 cells using transwell analysis. Cells invading through to the underside of the Matrigel-coated transwell membrane were fixed with ice-cold methanol for 30 minutes, stained with crystal violet and observed using an inverted microscope at 100x magnification. For each cell line, 10 hpfs were randomly chosen, the number of invaded cells per field was counted, and the total number of cells in the 10 hpfs was reported. Assays were performed in four replicates (inserts) for each cell line and repeated 3 times. Values were analyzed by one-way ANOVA and significant differences reported (P < .05). Data are expressed as mean ± SD.