(A) KB, KB-EFGP and KB-S100A8/A9 cells migrate to fill a uniform gap in the
monolayer. Cells were treated with Mitomycin-C before the motility assay to
inhibit cell proliferation. Monolayer closure expressed as a function of time
after removal of uniform swath of cells was observed, photographed, and measured
at times up to 48 h. The experiments were performed in triplicate for each cell
line and repeated at least three times with similar results. (B) Matrigel
invasion capacity of KB, KB-EFGP and KB-S100A8/A9 cells using transwell
analysis. Cells invading through to the underside of the Matrigel-coated
transwell membrane were fixed with ice-cold methanol for 30 minutes, stained
with crystal violet and observed using an inverted microscope at 100x
magnification. For each cell line, 10 hpfs were randomly chosen, the number of
invaded cells per field was counted, and the total number of cells in the 10
hpfs was reported. Assays were performed in four replicates (inserts) for each
cell line and repeated 3 times. Values were analyzed by one-way ANOVA and
significant differences reported (P < .05). Data are
expressed as mean ± SD.