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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Int J Biochem Cell Biol. 2014 Sep 16;55:279–287. doi: 10.1016/j.biocel.2014.09.007

Figure 3. Endogenous expression of MMP2 in TR146 cells.

Figure 3

(A) Confirmation of knockdown of endogenous S100A8 and S100A9 in TR146 cells using PCR analysis. (B) MMP2 mRNA levels in TR146, TR146-shRNA-control, TR146-S100A8/A9-shRNA cell lines determined using quantitative RT-PCR. All mRNA values were normalized to GAPDH, a housekeeping gene, and expressed relative to TR146 control cells. (C) Levels of MMP-2 protein in TR146, TR146-shRNA-control, and TR146-S100A8/A9-shRNA cells as determined using Western blots. Protein levels were normalized to β-actin and expressed relative to TR146 cells. Assays were performed in triplicate and repeated three times. The protein and mRNA levels were analyzed by one-way ANOVA and statistical differences between the cell lines were reported (P < 0.05).