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. 2014 Oct 12;5(19):8869–8878. doi: 10.18632/oncotarget.2588

Fig.1. Rluc-PCA design and quantification of cellular Myc-Max complexes.

Fig.1

(A) Schematic depiction of the Rluc-PCA based PPI reporter for the in vivo quantification of complex formation of Max and Myc proteins fused to the Rluc-PCA fragments 1 (F[1]) and 2 (F[2]), respectively. (B) The bHLH-LZ transcription factors Max (full length, aa 1-160) and Myc (full length, aa 1-439; or C-terminal fragment, aa 332-439) were fused at the C terminus to an interjacent 10-aa linker (GGGGS)2 and the Rluc-PCA fragments F[1] or F[2]. (C) Rluc-PCA signals were detected from resuspended HEK293 cells, transiently transfected with the indicated Rluc-PCA pairs and aliquoted to 96-well white-walled microtiter plates (representative experiment of n=3; ± SD of triplicates; RLU, relative light units). Rluc-PCA constructs based on PKA subunits (RII, PKAc) were used as controls.