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. 2014 Sep 3;5(19):8879–8892. doi: 10.18632/oncotarget.2432

Figure 4. ZMC1 reactivates additional p53 missense mutants with impaired zinc binding.

Figure 4

A, ZMC1 inhibits cell growth in zinc-binding p53 mutants. Mutations are labeled in the legend. B, ZMC1 induces a WT like conformational change in zinc binding mutants, shown by IF. The antibodies, PAB240 and PAB1620 are same as Figure 3E. Scale bar = 25 μm. C, ZMC1 induces expression of p53 regulated genes (p21 and PUMA) in zinc binding mutants, shown by quantitative RT-PCR. Relative gene expression level is normalized to β-actin. D, ZMC1 reduces mutant p53 stability in zinc binding mutants shown by western blot. The p53 protein level is detected by p53 antibody. β-actin is an internal control.