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. 2014 Aug 22;5(19):8893–8905. doi: 10.18632/oncotarget.2360

Figure 1. Silencing MUC1-C downregulates AKT and inhibits NSCLC cell growth.

Figure 1

(A) A549 cells were stably infected with lentiviruses expressing a control scrambled shRNA (CshRNA) or a MUC1 shRNA. Lysates were immunoblotted with the indicated antibodies. (B) Lysates from A549/CshRNA and A549/MUC1shRNA cells were immunoblotted with the indicated antibodies. (C) A549/CshRNA and A549/MUC1shRNA cells were plated at 5 × 104 cells/well. The results (mean±SD of three replicates) are expressed as cell number on day 4. (D) H460 cells were stably infected with lentiviruses expressing CshRNA or MUC1shRNA. Lysates were immunoblotted with the indicated antibodies. (E) Lysates from H460/CshRNA and H460/MUC1shRNA cells were immunoblotted with the indicated antibodies. (F) H460/CshRNA and H460/MUC1shRNA cells were plated at 5 × 104 cells/well. The results (mean±SD of three replicates) are expressed as cell number on day 4.