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. 2014 Sep 5;5(19):9382–9395. doi: 10.18632/oncotarget.2412

Figure 4. Phospho cofilin is attenuated by a LIM kinase inhibitor and Cucurbitacin I.

Figure 4

(A) Western blot analysis to assess the effect of LIMK1/2 inhibitor (BMS-5) on phospho cofilin (pCFL) and total cofilin at 10 uM and 20 uM. (B) Densitometric analysis using chemiluminescence to identify effect of BMS-5 on pCFL levels. pCFL was normalized to total CFL and a saturated blot is shown in A. (C) Western blot analysis to assess the effect of pCFL inhibitor (Cucurbitacin I) on phospho cofilin (pCFL) and total cofilin at 100 nM and 1 uM. (D) Densitometric analysis using chemiluminescence to identify effect of Cucurbitacin I on pCFL levels. pCFL was normalized to total CFL and a saturated blot is shown in C, *p<0.05.