Skip to main content
. 2014 Sep 8;5(19):9444–9459. doi: 10.18632/oncotarget.2452

Figure 7. miR-524-5p suppresses the tumorigenicity of melanoma cells in vivo.

Figure 7

(A) SK-Mel-19 cells harboring either the pre-mir-524-5p expression plasmid or control vector (Ctrl) were implanted in each thigh of a nude mouse. After 3 weeks, the tumor weight was measured. (Student's t- test: ** p< 0.01). (B) Total RNA was extracted from each tumor, and the expression levels of miR-524-5p were detected by qRT-PCR analysis. (C) and (D) The relative quantification results of protein levels of BRAF or ERK2 in each mouse tumor. The level of BRAF or ERK2 was normalized to GAPDH by ImageJ software analysis (Student's t- test: * p< 0.05). Western blotting was used to monitor the protein expression levels of BRAF, ERK1/2 and GAPDH in each tumor (Supplementary Figure 4).