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. 2014 Sep 9;5(19):9514–9529. doi: 10.18632/oncotarget.2413

Figure 1. Lung cancer cells fuse spontaneously with MSCs to form synkaryons with full chromosome complements.

Figure 1

(A) Strategy for isolating lung cancer cell-MSC fusion progeny by dual antibiotic selection and dual color FACS. (B) Flow cytometric analysis of GFP (FITC-A channel) and RFP (PE-A channel) in fusion progeny; percentages of dual GFP-RFP positive cells are shown in upper right quadrants of each graph. (C) Luciferase expression of fusion progeny and parental CB-MSC and H441 cell lines was imaged (below) and quantitated (above) with an IVIS Imaging System. (D) Merged fluorescence images of GFP (green) and DAPI (blue) demonstrate that fusion progeny are synkaryons. Scale bars represent 50 μm. (E) Representative metaphase spreads for karyotype analysis of fusion progeny and parent cell lines. (F) Box-and-whisker plots of chromosome numbers of fusion progeny and parental cell lines calculated by scoring karyotypes. Lines in boxes are medians, and maximum and minimum values are shown by the whiskers.