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. 2014 Sep 9;15(11):1175–1183. doi: 10.15252/embr.201439195

Figure 3. HDAC4 and HDAC4-regulated Lix1 regulate SC proliferation.

Figure 3

A–C Effects of HDAC4 KO on proliferation and apoptosis in SCs. Isolated SCs from 10-week-old male mice were cultured for 2 days in GM. Proliferation and apoptosis were monitored by staining cells with BrdU and caspase-3 antibodies, respectively. Approximately, 300 nuclei were measured per muscle. Scale bar: 200 μm. Columns, mean; bars, SEM. n = 3 for each group. **P < 0.01; NS, not significant, P > 0.05 (unpaired Student’s t-test).

D–F Effects of Lix1 knockdown (KD) on proliferation and apoptosis in SCs. Pooled SCs from muscles treated with CTX for 2 days in 8-week-old C57BL/6 male mice (10 mice) were transfected with Lix1 siRNAs and cultured for 2 days in GM. Medium was changed at 1 day after plating. Approximately 1,500 nuclei were counted per well. Scale bar: 200 μm. Columns, mean; bars, SEM. n = 3 for triplicate wells. **P < 0.01, ***P < 0.001 versus control siRNA no. 1 (unpaired Student’s t-test).