Skip to main content
. 2014 Nov 10;143(4):550–559. doi: 10.1111/imm.12333

Figure 2.

Figure 2

B cells from B-cell-specific NICD1-expressing mice were more activated by B-cell receptor (BCR) ligation than B cells from wild-type mouse. (a) B cells were isolated from the spleen of Cd19Cre/+, Cd19Cre/+ Notch1flox/flox and Cd19Cre/+ R26NICD1 mice. The isolated B cells were stimulated with anti-µ F(ab)2 or anti-µ F(ab)2 plus anti-CD40 antibodies for 16 hr. The activated cells were stained with fluorochrome-conjugated anti-CD69 and anti-CD86 antibodies and analysed by flow cytometry. (b) The summarized data from flow cytometry analyses are shown (± SD) for mean fluorescence intensity and percentage of gated populations. (c) The isolated B cells were stimulated with anti-µ F(ab)2 and anti-CD40 antibodies for 4 days. The cells were stained with a fluorochrome-conjugated anti-CD138 antibody and analysed by flow cytometry. (d) The isolated B cells were labelled with eFluor® 670 and stimulated with anti-µ F(ab)2 plus anti-CD40 antibodies for 4 days. Cells were analysed by flow cytometry. Data are representative of three independent experiments. Student’s t-test: *P < 0·05; **P < 0·01.