Figure 6.

Notch1 deficiency decreases in B-cell antibody secretion. (a–e) B cells were isolated from the spleens of Cd19^Cre/+ and Cd19^Cre/+Notch1^flox/flox mice and stimulated by anti-µ F(ab)₂ plus anti-CD40 antibodies for 5 days with or without OP9-Dll1 cells and secreted IgM (a), IgG2a(b), IgG2b(c) or IgG3(d), or IgG1(e) levels were analysed by ELISA. (f) Analysis of antigen-specific antibody production in response to the T-cell independent antigens NP-Ficoll (TI type 2) or with NP-LPS (TI type 1) and TD antigen (NP-CGG). Data are representative of two independent experiments. Student’s t-test: * P < 0·05; **P < 0·01.