Figure 6.

Herpes simplex virus type 1 (HSV-1) induces up-regulation of MHC-I, but not stress molecules MICA/B. (a) Fold increase in HLA-ABC or (b) HLA-E expression as analysed by flow cytometry after exposure of purified monocytes to HSV_UV, HSV_INF, HSV_GFP, and HSV_d106S for 24 and 48 hr. **P < 0·01 compared with HSV_UV (Tukey HSD). Results are representative of eleven (HLA-ABC) and three (HLA-E) independent experiments. (c) Interferon-α (IFN-α) production after exposure of purified monocytes to HSV_UV, HSV_INF, and HSV_GFP for 24 and 48 hr. Data represent eight independent experiments. (d) HLA-ABC expression on monocytes exposed to HSV_GFP in the presence of a neutralizing IFN-α receptor antibody (aIFN-aR). Data represent three independent experiments. *P < 0·05, **P < 0·01 compared with the isotype control (Student’s t-test). All merged data are presented as mean and standard error. (e) Representative example of GFP and HLA-ABC- and HLA-E expression in monocytes exposed to HSV_GFP in the presence of aIFN-aR or IgG2a isotype. (f) Representative example for MICA/B expression on isolated monocytes 24 hr post stimulation.