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. 2014 Nov 10;143(4):670–678. doi: 10.1111/imm.12350

Figure 6.

Figure 6

Silencing A20 rescues myeloid dendritic cell (mDC) interleukin-12 (IL-12) suppression and decreases IL-10 production in mDCs from treatment-naive patients. Myeloid DCs transfected with A20 small interfering RNA (siRNA) or control siRNA were subjected to RT-PCR and Western blot detection of A20 expression and ELISA of IL-12p70 and IL-10 secreted into the culture supernatant by mDCs. A20 siRNA inhibited hepatitis C virus (HCV) infection induced A20 expression by RT-PCR (a) and Western blot detection (b), a stronger silencing of A20 protein was achieved 48 hr and 72 hr after transfection. HCV-mediated IL-12 inhibition was partially restored (c) and the IL-10 production was decreased (d) in mDCs transfected with A20 siRNA. The data were reproducible in three repeated experiments.**P < 0·01, ***P < 0·001.