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. 2014 Aug 4;33(20):2295–2313. doi: 10.15252/embj.201387500

Figure 8. Hh and Yki regulate niche competition and self-renewal independently.

Figure 8

A–C yki mutant CySCs did not self-renew (A) and loss of yki suppressed the ptc colonization phenotype (C). Testes with negatively marked yki (A), ptc (B), or ptc yki double mutant (C) clones at 7 dpci. Absence of GFP (single channel A’, B’, C’) marks the clones, Vasa (single channel A”, B”, C”) is red, and Tj (single channel A”’, B’”, C’”) is blue. Arrows point to differentiated mutant cyst cells in (A) and (C).

D ptc mutant clones (arrow) did not display increases in ex-lacZ expression compared to wild type (arrowhead). Clones are labeled by GFP (single channel D’), β-gal is in red (single channel D”), and Zfh1 is in blue (single channel D’”). The hub is outlined with a dotted line in all panels.

E Graph of clone recovery rates over time for the indicated genotypes. n = 61, 21, 88 for control at 2, 7, 14 dpci, respectively; n = 46, 39, 89 for ptc at 2, 7, 14 dpci, respectively; n = 50, 94, 35 for yki at 2, 7, 14 dpci, respectively; n = 40, 59, 64 for ptc yki at 2, 7, 14 dpci, respectively; n = 39, 67 for hpo at 2, 14 dpci, respectively.

F yki mutant GSCs could be recovered at 2 and 7 dpci. yki mutant CySCs could be recovered at 2 dpci but not 7 dpci. n = 61, 21 for control at 2, 7 dpci, respectively. n = 50, 94 for yki at 2, 7 dpci, respectively.