Figure 2. Akirin2 is critical for innate immune responses to Listeria infection in vivo.

LysM-Cre⁺; Akirin2^fl/+ and LysM-Cre⁺; Akirin2^fl/fl mice (n = 7) were intraperitoneally infected with 5 × 10⁵ Listeria monocytogenes/mice in a volume of 0.25 ml of PBS.

A Serum cytokine levels were determined by ELISA for IL-6, TNF, and IL-12p40 at indicated time periods following infection.

B PECs were prepared from LysM-Cre⁺; Akirin2^fl/+ (n = 4) and LysM-Cre⁺; Akirin2^fl/fl (n = 5) mice 24 h after Listeria infection, and the cell surface expression of myeloid cell markers was determined by flow cytometry. Frequencies of CD11b⁺Ly6G^hi neutrophils and CD11b⁺Ly6C^hiLy6G^lo inflammatory monocytes are indicated.

C, D The numbers of (C) total PECs, CD11b⁺ myeloid cells and (D) CD11b⁺Ly6C^hiLy6G^lo inflammatory monocytes and CD11b⁺Ly6G^hi neutrophils are shown.

E The levels of Il6 and Tnf mRNAs in the PECs were measured by qPCR.

F The bacterial load in the liver and spleen was measured by colony formation assay, and the number of bacterial colonies was then counted and expressed as CFU/organ (n = 4).

Data information: Error bars indicate mean ± s.d. Statistical significance was determined using the Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.