A, B WISH for runx1 in 26 hpf hsp70:gal4; UAS:NICD-myc uninjected or notch3 morphant transgenic embryos with heat-shock induction at 14 hpf (A) or 20 hpf (B), with or without enforced NICD expression. Arrowheads indicate the presence or absence of HSPCs at the midline.
C Quantitation of results recording percentages of embryos displaying normal or decreased numbers of runx1+ HSPCs at 26 hpf in notch3 morphants with heat-shock induction conditions.