A, B WISH for runx1 in 26 hpf kdrl:gal4 (A) or phldb1:gal4-mcherry (B) crossed to UAS:NICD-myc transgenic embryos either uninjected or injected with notch3 morpholino, with or without enforced NICD expression. Arrowheads indicate the presence or absence of HSPCs at the midline.
C Quantitation of results recording percentages of embryos displaying normal or decreased numbers of runx1+ HSPCs at 26 hpf in notch3 morphants with tissue-specific induction conditions.