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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Pain. 2014 Aug 29;155(11):2344–2359. doi: 10.1016/j.pain.2014.08.034

Fig. 9.

Fig. 9

Nociceptive stimulation increased caspase 8 mRNA and protein expression

A) Caspase 8 mRNA was unaltered by SCI or SCI+STIM in the acute time points. At 7 days and in the ventral spinal cord at 28 days, caspase 8 mRNA was increased in both SCI and SCI+STIM groups. At 28 days, in the dorsal spinal cord of SCI+STIM subjects, caspase 8 mRNA levels was significantly increased relative to sham controls and SCI. B) Western blot analyses showed that noxious stimulation significantly increased caspase 8 protein levels at 24 hours. At the later time points, dorsal spinal cord levels of caspase 8 were significantly up-regulated in SCI and SCI+STIM subjects compared to shams, while ventral levels were unchanged. [* indicates p < .05 relative to the sham control group, and # indicates p < .05 of the SCI+STIM group relative to the SCI group]. (C) Examples of representative western blot images for caspase 8. β-tubulin served as a loading control protein.