Table 1.
Top upregulated and downregulated transcripts in WT and BK5.EP4 mice.
| Gene | Gene name | log2 ratio | p‐value | Fold change |
|---|---|---|---|---|
| A. Top upregulated and downregulated transcripts for PGE 2 treated EP4 relative to WT mice | ||||
| Upregulated | ||||
| A130040M12Rik | Unclassified gene; VL30 family | 1.60859 | 5.20E‐06 | 3.05 |
| IL‐20 | Interleukin‐20 | No transcript | 2.33E‐07 | |
| Kcnk2 | Potassium channel, subfamily K, member 2; Trek1 | 2.32131 | 1.90E‐05 | 4.99 |
| Krtap16‐10b | Keratin associated protein 16‐10b | No transcript | 4.09E‐05 | |
| Ptger4 | Prostaglandin E receptor 4 (EP4) | 1.34283 | 7.99E‐06 | 2.54 |
| Serpinb6d | Serine (or cysteine) peptidase inhibitor, clade B member 6d | 2.11551 | 7.33E‐07 | 4.33 |
| Sprr4 | Small proline‐rich protein 4 | 1.40363 | 9.13E‐08 | 2.65 |
| Downregulated | ||||
| Akr1c13 | Aldo‐keto reductase family 1, member C13 | −2.42942 | 2.32E‐05 | 0.19 |
| Akr1c19 | Aldo‐keto reductase family 1, member C19 | −3.51993 | 3.74E‐05 | 0.09 |
| Cartpt | Cocaine‐and amphetamine‐regulated prepropeptide | −6.54051 | 1.76E‐05 | 0.01 |
| Hamp | Hepcidin antimicrobial peptide | −9.58007 | 1.50E‐05 | 0.04 |
| S100a8 | S100 calcium binding protein A8 | −2.73713 | 3.10E‐05 | 0.21 |
| Scd3 | Stearoyl‐coenzyme A desaturase 3 | −2.00069 | 4.03E‐05 | 0.25 |
| Steap | Six‐transmembrane epithelial antigen of prostate 4 | −2.25736 | 7.12E‐17 | 0.21 |
| B. Top upregulated and downregulated transcripts: EP4 PGE 2 relative to EP4 EtOH | ||||
| Upregulated | ||||
| Csf2rb | Colony stimulating factor 2 receptor beta | 1.33980634 | 3.94E‐06 | 2.53 |
| Mt4 | Metallothionein 4 | 1.21674309 | 9.05E‐07 | 2.32 |
| Pmepa1 | Prostate transmembrane protein, androgen induced 1 | 1.66276986 | 2.04E‐05 | 3.17 |
| Downregulated | ||||
| C230091D08Rik | Unclassified non‐coding RNA gene | −1.2859205 | 9.22E‐06 | 0.41 |
| Coro2a | Coronin, actin binding 2A | −1.9046218 | 1.27E‐05 | 0.27 |
| Gm9705 | Cytochrome P450 family; CYP4f37 | −2.3798049 | 1.32E‐07 | 0.19 |
| Mill1 | MHC I like leukocyte 1 | −1.340297 | 4.78E‐07 | 0.39 |
| Mmd | Monocyte to macrophage differentiation‐associated | −1.368169 | 1.31E‐05 | 0.39 |
| Slc39a6 | Solute carrier family 39 (metal ion transporter), member 6 | −1.226609 | 3.38E‐05 | 0.43 |
| Smoc2 | SPARC related modular calcium binding 2 | −1.632054 | 8.69E‐08 | 0.32 |
| Steap4 | Six‐transmembrane epithelial antigen of prostate, member 4 | −1.403364 | 8.15E‐08 | 0.38 |
| C. IL‐20 protein concentrations 8 h after treatment of WT and BK5.EP4 mice with EtOH or PGE2, as measured by ELISA | ||||
| Genotype | Treatment | x ± sd | ||
| WT | EtOH | 290.8 ± 1.12 | ||
| WT | PGE2 | 438.6 ± 30.28 | ||
| BK5.EP4 | EtOH | 378.3 ± 36.83 | ||
| BK5.EP4 | PGE2 | 1297.8 ± 135.36 | ||
Values represent the mean (n = 3–5 mice) ± std dev (pg IL‐20/?g protein). WT‐PGE2 differs significantly from WT‐EtOH (p < 0.001); BK5.EP4‐PGE2 differs significantly from BK5.EtOH and both WT groups (p < 0.01).