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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Dev Biol. 2014 Sep 30;396(1):150–157. doi: 10.1016/j.ydbio.2014.09.024

Fig. 3. Disruption of nhr-1 prevents development of accessory reproductive organs.

Fig. 3

(A–C′) Whole-mount, colorimetric in situ hybridization to detect tsp-1 in the cement glands (g), grn in the sperm ducts (sd) and seminal vesicles (sv), and eya in the oviducts (od) in adult sexual animals after being fed a control double-stranded RNA. (D–F′) In situ hybridization for tsp-1, grn, and eya after being fed nhr-1 double-stranded RNA. Expression of these genes is not detected. The gonopore (gp) is also missing from nhr-1 knockdown animals. Ventral view shown for all animals. Data are representative of three individual planarians. Scale bars: (A–F) 500 μm. (A′–F′) 200 μm.