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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Gastroenterology. 2014 Aug 27;147(6):1363–1377.e17. doi: 10.1053/j.gastro.2014.08.033

Figure 1. Generation and verification of mice with tissue specific TLR5 deletion.

Figure 1

(A) Schematical representation of the transgene containing TLR5 exon 1 flanked with LoxP sites. (B) Analysis of TLR5 mRNA expression by q-RT-PCR in blood, ling, liver, spleen, kidney, stomach, duodenum, jejunum, ileum, caecum, colon, colonic mucosa and purified CD11c+ splenic cells. (C) Analysis of CXCL1 mRNA expression by q-RT-PCR in the colonic mucosa and CXCL1 protein expression by ELISA in serum. Mice were intra-peritoneally injected with 20μg flagellin (+) or PBS (−). 30 min later, serum and intestinal mRNA were isolated. (D) Analysis of IL22 mRNA expression by q-RT-PCR in the colon and IL22 protein expression by ELISA in the serum of mice treated or not with 20μg of flagellin for 2 hours. Data are the means +/− S.E.M. or points are from individual mice with bar representing the mean. Significance was determined by Student's t-test, * indicates p<0.05 compared to TLR5fl/fl group.